| Interaction ID | Mol A | Type | Species | Verb | Nature | Mol B | Type | Species |   |
|---|
|
| 5693 | CCL2 | Protein | Homo sapiens-e | Bind | direct | CCR2 | Protein | Homo sapiens-e | | | Monomeric monocyte chemoattractant protein-1
(MCP-1) binds and activates the MCP-1 receptor CCR2B. /
These data suggest that MCP-1 binds and activates its
receptor as a monomer. In contrast, Y13A*, another monomeric
mutant, has a 100-fold weaker binding affinity, is a much
less potent inhibitor of adenylate cyclase and stimulator of
calcium influx, and is unable to stimulate chemotaxis. Thus
Tyr13 may make important contacts with the receptor that are
required for high affinity binding and signal transduction. | | | |
Interaction id | 5693 | |
MOLECULE A | |
Id | 6347 |
Type | Protein |
Species | Homo sapiens-e | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | 729230 |
Type | Protein |
Species | Homo sapiens-e | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | | Kinetics | - | |
|
General Information |
Interaction term | Bind (
direct ) | |
Pathway | Cytokine And Chemokine Mediated Signaling Pathway | |
Comments | |
Domain_motif_site_residue | | (PB021356)[RES:Tyr13](6347) |
| |
References | |
PubMed Id | 9837883 | |
Author | Paavola CD, Hemmerich S, Grunberger D, Polsky I, Bloom A,
Freedman R, Mulkins , M, Bhakta S, McCarley D, Wiesent L, Wong
B, Jarnagin K, Handel TM | |
Title | Monomeric monocyte chemoattractant protein-1 (MCP-1) binds
and activates the MCP-1 receptor CCR2B. |
|
|
| | |
| 5694 | CCL2 | Protein | Homo sapiens-e | Increases activity | direct | CCR2 | Protein | Homo sapiens-e | | | Monomeric monocyte chemoattractant protein-1
(MCP-1) binds and activates the MCP-1 receptor CCR2B. /
These data suggest that MCP-1 binds and activates its
receptor as a monomer. In contrast, Y13A*, another monomeric
mutant, has a 100-fold weaker binding affinity, is a much
less potent inhibitor of adenylate cyclase and stimulator of
calcium influx, and is unable to stimulate chemotaxis. Thus
Tyr13 may make important contacts with the receptor that are
required for high affinity binding and signal transduction. | | | |
Interaction id | 5694 | |
MOLECULE A | |
Id | 6347 |
Type | Protein |
Species | Homo sapiens-e | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | 729230 |
Type | Protein |
Species | Homo sapiens-e | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | | Kinetics | - | |
|
General Information |
Interaction term | Increases activity (
direct ) | |
Pathway | Cytokine And Chemokine Mediated Signaling Pathway | |
Comments | |
Domain_motif_site_residue | | (PB021356)[RES:Tyr13](6347) |
| |
References | |
PubMed Id | 9837883 | |
Author | Paavola CD, Hemmerich S, Grunberger D, Polsky I, Bloom A,
Freedman R, Mulkins , M, Bhakta S, McCarley D, Wiesent L, Wong
B, Jarnagin K, Handel TM | |
Title | Monomeric monocyte chemoattractant protein-1 (MCP-1) binds
and activates the MCP-1 receptor CCR2B. |
|
|
| | |
| 210685 | CCR2 | Protein | Homo sapiens-e | Increases activity | indirect | MAPK1 | Protein | Homo sapiens | | | Within 2 min, the MCPs (1-4) elicited a rapid
and transient activation of MAPK in peripheral blood
mononuclear cells and in HEK-293 cells expressing CCR2b.
U0126, an inhibitor of MAPK-kinase (MEK) activation, not
only prevented extracellular signal-regulated kinase 1/2
(ERK1/2) activation but also significantly inhibited the
MCP-mediated chemotaxis. | | | |
Interaction id | 210685 | |
MOLECULE A | |
Id | 729230 |
Type | Protein |
Species | Homo sapiens-e | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | 5594 |
Type | Protein |
Species | Homo sapiens | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | |
Regulator | |
Regulation | Positive | | Description | Ligand of molecule A:CCL2 |
| |
Regulation | Negative | |
| | Kinetics | | Mol B | | Conc | Time | Others | Comments | | | | Rapid
Transient
|
| | Regulators | | Name | Effect | Conc | Time | Others | Comments | | CCL2 | Stimulant | | 2min
| | |
|
| |
|
General Information |
Interaction term | Increases activity (
indirect ) | |
Pathway | Cytokine And Chemokine Mediated Signaling Pathway | |
Interaction result | |
Other Resultant | | Chemotaxis |
| |
Experimental location and method | - species::Human
- cell::HEK293 cells
| |
Comments | |
General | | Also seen in peripheral blood mononuclear cells |
| |
References | |
PubMed Id | 11966759 | |
Author | Wain JH, Kirby JA, Ali S | |
Title | Leucocyte chemotaxis: Examination of mitogen-activated
protein kinase and phosphoinositide 3-kinase activation by
Monocyte Chemoattractant Proteins-1, -2, -3 and -4. |
|
|
| | |
| 232119 | CCR2 | Protein | Homo sapiens-e | Increases activity | indirect | MAPK3 | Protein | Homo sapiens | | | Within 2 min, the MCPs (1-4) elicited a rapid
and transient activation of MAPK in peripheral blood
mononuclear cells and in HEK-293 cells expressing CCR2b.
U0126, an inhibitor of MAPK-kinase (MEK) activation, not
only prevented extracellular signal-regulated kinase 1/2
(ERK1/2) activation but also significantly inhibited the
MCP-mediated chemotaxis. | | | |
Interaction id | 232119 | |
MOLECULE A | |
Id | 729230 |
Type | Protein |
Species | Homo sapiens-e | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | 5595 |
Type | Protein |
Species | Homo sapiens | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | |
Regulator | |
Regulation | Positive | | Description | Ligand of molecule A:CCL2 |
| |
Regulation | Negative | |
| | Kinetics | - | |
|
General Information |
Interaction term | Increases activity (
indirect ) | |
Pathway | Cytokine And Chemokine Mediated Signaling Pathway | |
Interaction result | |
Other Resultant | | Chemotaxis |
| |
Experimental location and method | - species::Human
- cell::HEK293 cells
| |
Comments | |
General | | Also seen in peripheral blood mononuclear cells |
| |
References | |
PubMed Id | 11966759 | |
Author | Wain JH, Kirby JA, Ali S | |
Title | Leucocyte chemotaxis: Examination of mitogen-activated
protein kinase and phosphoinositide 3-kinase activation by
Monocyte Chemoattractant Proteins-1, -2, -3 and -4. |
|
|
| | |
| 400058 | CCL2 | Protein | Homo sapiens-e | Increases expression | indirect | CCR2 | Protein | Homo sapiens | | | Here we show that cultured human fetal
astrocytes express CCR2 at the mRNA and protein levels, and
display chemotaxis and calcium flux in response to MCP-1.
Surface CCR2 protein expression and MCP-1 binding activity
were observed to undergo near parallel downmodulation and
recovery following MCP-1 exposure, supporting the argument
that CCR2, and not another receptor, mediates MCP-1 ligation
in these cells. | | | |
Interaction id | 400058 | |
MOLECULE A | |
Id | 6347 |
Type | Protein |
Species | Homo sapiens-e | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | 729230 |
Type | Protein |
Species | Homo sapiens | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | | Kinetics | - | |
|
General Information |
Interaction term | Increases expression (
indirect ) | |
Experimental location and method | - Location context::Interaction specific
- species::Human
- cell / cell line::Astrocytes
- primary::N
- condition::Fetal astrocytes
| |
References | |
PubMed Id | 12271471 | |
Author | Andjelkovic AV, Song L, Dzenko KA, Cong H, Pachter JS | |
Title | Functional expression of CCR2 by human fetal astrocytes. |
|
|
| |
|
d: for diseases
Structure