| Interaction ID | Mol A | Type | Species | Verb | Nature | Mol B | Type | Species |   |
|---|
|
| 176538 | Retinoate | Small molecule | Small molecule | Decreases expression | indirect | TNF | Protein | Homo sapiens | | | Because vitamins preserving anti-oxidative
effects are suggested to provide protection in OA patients
from joint damage, in the present study, we examined the
effects and mechanisms of all-trans retinoic acid (t-RA) in
suppressing pro-inflammatory cytokine-induced matrix
metalloproteinases (MMPs) production in human chondrocytes.
Chondrocytes were prepared from cartilage specimens of OA
patients receiving total hip or total knee replacement. The
protein concentration was measured by ELISA, the mRNA
expression by reverse transcriptase-polymerase chain
reaction, the protein expression by Western blotting, the
transcription factor DNA-binding activity by electrophoretic
mobility shift assay and the protein kinase activity by
kinase assay. / Furthermore, we showed that t-RA could
reduce IL-1-induced TNF-alpha production in chondrocytes. | | | |
Interaction id | 176538 | |
MOLECULE A | |
Id | 302-79-4 |
Type | Small molecule |
Species | Small molecule | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | 7124 |
Type | Protein |
Species | Homo sapiens | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | | Kinetics | - | |
|
General Information |
Interaction term | Decreases expression (
indirect ) | |
Pathway | Ligand-dependent nuclear receptor
activity:Retinoic acid receptor activity | |
Disease Details | | |
Experimental location and method | - Experimental method::Blotting, Western
- species::Human
- condition::Interleukin 1 treated
chondrocytes prepared from cartilage specimens of
osteoarthritis patients receiving total hip or total
knee replacement
- cell::Chondrocytes
| |
References | |
PubMed Id | 15946654 | |
Author | Ho LJ, Lin LC, Hung LF, Wang SJ, Lee CH, Chang DM, Lai JH,
Tai TY | |
Title | Retinoic acid blocks pro-inflammatory cytokine-induced matrix
metalloproteinase production by down-regulating JNK-AP-1
signaling in human chondrocytes. |
|
|
| |
|
d: for diseases
Disease