| Interaction ID | Mol A | Type | Species | Verb | Nature | Mol B | Type | Species |   |
|---|
|
| 166347 | Phospholipase C | Family | Rattus norvegicus | Increases level | indirect | Inositol 3-phosphate | Small molecule | Small molecule | | | Stimulation of inositol phosphate formation
in ROS 17/2.8 cell membranes by guanine nucleotide, calcium,
and parathyroid hormone. / The nonhydrolyzable guanine
nucleotide, GTP gamma S, and calcium ion, alone and
together, stimulated all three IPs, IP1, IP2, and IP3. IP1
formation was linear over 30 minutes but IP2 and IP3
accumulated more rapidly peaking by 5 minutes for all
agonist conditions. / The results demonstrate that inositol
phosphate formation can be stimulated directly in a membrane
preparation of ROS cells by GTP gamma S, calcium ion, and
PTH and that the enzyme mediating this activity,
phospholipase C, is regulated by a guanine nucleotide
binding protein. | | | |
Interaction id | 166347 | |
MOLECULE A | |
Id | EZRnF00011 |
Type | Family |
Species | Rattus norvegicus | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | Inositol 3-phosphate |
Type | Small molecule |
Species | Small molecule | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | | Kinetics | - | |
|
General Information |
Interaction term | Increases level (
indirect ) | |
Pathway | Second-Messenger-Mediated Signaling:cGMP-Mediated SignalingHydrolase activity:Phospholipase C activityHormone-Mediated SignalingSecond-Messenger-Mediated
Signaling:Calcium-Mediated Signaling | |
Experimental location and method | - species::Rat
- condition::PTH or Calcium(ion or
Guanosine 5'-O-3-Thiotriphosphate treated cells
- cell::ROS17/2.8 cells
- sub cellular::Plasma membrane
| |
References | |
PubMed Id | 2763877 | |
Author | Cosman F, Morrow B, Kopal M, Bilezikian JP | |
Title | Stimulation of inositol phosphate formation in ROS 17/2.8
cell membranes by guanine nucleotide, calcium, and parathyroid hormone. |
|
|
| | |
| 189260 | Calcium | Small molecule | Small molecule | Increases activity | indirect | Phospholipase C | Family | Homo sapiens-e | | | Neoplastic keratinocyte cell lines expressing
a mutant c-Ha-ras gene and normal keratinocytes transformed
to the neoplastic phenotype by transduction with the
v-Ha-ras gene (v-Ha-ras keratinocytes) have elevated
constitutive activity of PLC that increases further in
response to Ca2+, but the cells do not differentiate normally. | | | |
Interaction id | 189260 | |
MOLECULE A | |
Id | 7440-70-2 |
Type | Small molecule |
Species | Small molecule | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | EZHsF00011 |
Type | Family |
Species | Homo sapiens-e | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | | Kinetics | - | |
|
General Information |
Interaction term | Increases activity (
indirect ) | |
Pathway | Second-Messenger-Mediated
Signaling:Calcium-Mediated Signaling | |
Disease Details | | |
Experimental location and method | - condition::Normal keratinocytes
transformed to the neoplastic phenotype by
transduction with the v-Ha-ras gene
- cell::Keratinocytes
| |
References | |
PubMed Id | 8068182 | |
Author | Punnonen K, Denning MF, Rhee SG, Yuspa SH | |
Title | Differences in the regulation of
phosphatidylinositol-specific phospholipase C in normal and
neoplastic keratinocytes. |
|
|
| | |
| 430063 | Phospholipase C | Protein | Homo sapiens | Increases mobilization | indirect | Calcium | Small molecule | Small molecule | | | PAR2 agonists increased intracellular [Ca2+]
([Ca2+]i) in these neurons in culture, and PAR2-responsive
neurons also responded to the TRPV1 agonist capsaicin,
confirming coexpression of PAR2 and TRPV1. PAR2 agonists
potentiated capsaicin-induced increases in [Ca2+]i in
TRPV1-transfected human embryonic kidney (HEK) cells and DRG
neurons and potentiated capsaicin-induced currents in DRG
neurons. / Inhibitors of phospholipase C and protein kinase
C (PKC) suppressed PAR2-induced sensitization of
TRPV1-mediated changes in [Ca2+]i and TRPV1 currents. | | | |
Interaction id | 430063 | |
MOLECULE A | |
Id | EZHsF00011 |
Type | Protein |
Species | Homo sapiens | |
Attribute |
--
|
Structure Details | -- |
Disease Details | -- | |
MOLECULE B | |
Id | Calcium |
Type | Small molecule |
Species | Small molecule | |
Attribute | -- |
Structure Details | -- |
Disease Details | -- | |
Regulator | |
Id | 7442 : TRPV1 |
Type | Protein |
Species | Homo sapiens-e | | Induce | | Attribute | Description | | Activated:
| by capsaicin |
|
|
Structure Details | |
Disease Details | | |
Id | 404-86-4 : Capsaicin |
Type | Small molecule |
Species | Small molecule | |
Structure Details | |
Disease Details | | |
Id | 2150 : F2RL1 |
Type | Protein |
Species | Homo sapiens | | Stimulate | | Attribute | Description | | Activated:
| by agonist |
|
|
Structure Details | |
Disease Details | | | Kinetics | - | |
|
General Information |
Interaction term | Increases mobilization (
indirect ) | |
Experimental location and method | - Location context::Interaction specific
- species::Human
- cell / cell line::293
- primary::N
- Location context::Supporting evidence
- organ / tissue::Spinal Ganglia
| |
Comments | |
General | | Referred full text for location-293 cells |
| |
References | |
PubMed Id | 15128844 | |
Author | Amadesi S, Nie J, Vergnolle N, Cottrell GS, Grady EF,
Trevisani M, Manni C, Geppetti P, McRoberts JA, Ennes H, Davis
JB, Mayer EA, Bunnett NW | |
Title | Protease-activated receptor 2 sensitizes the capsaicin
receptor transient receptor potential vanilloid receptor 1 to
induce hyperalgesia. |
|
|
| |
|
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