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Ku antigen, RBP-Jk and p50 were found to bind
to the DNA region containing the kB element in the p50
promoter. / mRNA expression and de novo synthesis of p50
were inhibited in cells transfected with the mutated gene
expression constructs for IkBa, Ku80 and RBP-Jk. A reporter
assay demonstrated that p50 transcription was positively
mediated by NF-kB, Ku antigen and RBP-Jk, and that the
binding elements for these proteins are required for optimal
p50 expression.